Simultaneous dyeing and antibacterial finishing of polypropylene using vinyl sulfone dye under supercritical carbon dioxide

Polypropylene fibres are difficult to dye using commonly used techniques due to the high crystallinity and non-polar aliphatic structure, that lack reactive places for dyes in the molecule. Dyeing PP fabric in scCO2 with antibacterial dyes merged the dyeing and finishing methods, resulting in a more productive technique in terms of water and energy consumption. Unmodified polypropylene fabric was dyed with 4-[2-[4-(ethenylsulphonyl)phenyl]diazenyl]-N,N-diethylbenzenamine antibacterial dye under scCO2 medium. The influences of scCO2 working parameters, such as dye concentration, pressure, dyeing time, and temperature, on fabric dye absorption expressed as color strength were studied. The color strength (K/S) was measured as well as CIELAB color parameters. The results were compared with its water dyeing analogue and it was observed that color strength as well as color depth (L) of the samples dyed in scCO2 were noticeably better than its water counterpart. In both scCO2 and water, the fastness properties (washing, rubbing, and light) of the dyed samples were excellent. Antibacterial activity of the dyed polypropylene sample in scCO2 was estimated and the results indicated good antibacterial efficiency.


Scientific Reports
| (2022) 12:8789 | https://doi.org/10.1038/s41598-022-12680-w www.nature.com/scientificreports/ strength of the dyed PP fibres in scCO 2 was found to be higher than in water. The fastness properties (washing, light, rubbing) of the dyed samples under study in scCO 2 and water were excellent 31 . Sulfone derivatives provide an example of an important class of bioactive compounds. Literature described sulfone as anti-fungal 32 , antiinflammatory 33 , anti-HIV 34 , anti-tubercular 35 , anti-cancer 36 , anti-hepatitis 37 and anti-tumor 38 agents. In continuation of our work, we report here on a one-step dyeing and antibacterial finishing of PP fabrics with synthesized disperse reactive dye containing sulfonyl functional group under scCO 2 medium.

Experimental sections
Materials and dyes. Dyes. The orange antibacterial vinyl sulfone disperse reactive dye ( Fig. 1) was produced using the method previously mentioned and was prepared for use in scCO 2 39 .
Material. The dyeing substrate was a 100% unmodified polypropylene fabric provided by Shikisen-sha Company (Osaka, Japan). Crystallinity of polypropylene fabrics is calculated using Eq. (1) Δ H = melting enthalpy: 105.8 J/ g, Δ Hm = ideal crystal melting enthalpy: 209.1 J/g (Brandrup, 1999). Crystallinity: 50.6% Water dyeing apparatus. Infra color dyeing system was used for water dyeing. It was composed of beakers mounted on a circular beaker carrier wheel. Heating with infrared radiation, cooling with air, and automation with the DC4 F/R microprocessor programmer. The maximum temperature reached 140 °C, with a maximum average heating rate of 5 °C/min and a maximum cooling rate of 3 °C/min. Supercritical carbon dioxide dyeing apparatus. As shown in (Fig. 2), the main components of a scCO 2 apparatus are: CO 2 cylinder, a chiller (model Julabo FL601), an extraction RHPLC pump (model JASCO PU-4180), a semi-preparative CO 2 pump (model JASCO PU-4386), a heater controller (model HC-2068-01), and a back pressure regulator (model JASCO BP-4340) with a maximum rate of pressure of 30 MPa, a temperature controller and speed controller (model EYELARCX-1000 H) with a maximum temperature rate of 130 °C, a dyeing autoclave with an internal capacity of approximately 50 mL, and a maximum CO 2 flow rate of a circulation pump of 10 mL/min,    liquor-to-goods ratio of 1:50 was used for the dyeing experiment. The dyeing was performed at a concentration of 2% dye (based on the fabric weight). Then, in the dyeing machine, a PP fabric (10 × 10 cm) was inserted. Over the duration of 45 min, the temperature was increased from 35 °C to 130 °C, and dyeing was continued at that temperature for around 1 h. The samples were dyed, then washed in a bath containing 2% nonionic detergent (Sera Fast C-RD) at 60 °C, rinsed, and dried at room temperature.
Dyeing unmodified PP fabrics in scCO 2 . In the beginning, (CO 2 ) gas was cooled to -5 °C by the chiller. PP fabric (3 × 10 cm) was covered by the screw tube and was inserted in the dyeing vessel. The dye was added to the bottom of the vessel's floor, and the concentration of dyestuff used ranged from 1 to 3%. (owf). Liquid CO 2 was transported from the valve to the entire system by the semi-preparative CO 2 pump. The autoclave was sealed and heated until the system reached the desired pressure (15,20,250 MPa), temperature (100, 120, 130 °C), and dyeing time (1, 2, 3 h) in line with the study of dyeing in scCO 2 . The shut-off valve was slowly opened to release CO 2 until the pressure in the dyeing vessel reduced and reached atmospheric pressure automatically. The dyed sample was removed from the vessel. To eliminate the remaining dyestuff on the surface of the dyed samples, they were immersed in a bath containing 2% nonionic detergent (Sera Quick CRD) at 60 °C, rinsed with water, and dried at room temperature.
Measurements and testing analysis. Mechanical testing of unmodified PP fabrics. The mechanical properties of undyed and dyed PP fabrics were tested using a Tinius Olsen with a load cell 500 N, preload 0.01 N, speed 100 mm/min, and gauge length 100 mm in both scCO 2 and water medium.
Antibacterial activity. Antibacterial activity assessment against Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and expressed as zone of growth inhibition ZI (mm). The antibacterial activity was determined by the agar well diffusion method 12 .
Raman spectra of PP fabrics. Using the Jasco NRS-4500, the Raman spectra of undyed and dyed PP fabrics under scCO 2 were observed in the range of 200 to 4000 cm -1 .

Statistical analysis.
All of the experiments were completed by averaging three (sample) readings. The standard error of the mean was determined using the Eq. (4) below, and it was discovered to be + ( −) 0.1 where SE is standard error, S is sample standard deviation, and n is the number of observations of the sample.

Results and discussion
Factors affecting dyeing properties of unmodified PP fabrics dyed in scCO 2 medium. The influences of scCO 2 working conditions, such as dye concentration, system pressure, dyeing time, and temperature were studied as influencing factors 40,41 . Values of the K/S of dyed samples were carried out in the range of (360-490 nm). 4%), the effect of dye concentration on (K/S) of PP fabric dyed in scCO 2 was investigated. The dyeing pressure was set to 25 MPa, the temperature was set to 130 °C, the dyeing time was set to 60 min, and the dyeing medium flow rate was set to 7.500 ml/min during this procedure. As was revealed in (Fig. 3), the K/S of dyed samples in scCO 2 was increased from 4.55 to 7.22 and it became constant at 4%. PP fibers have a high number of non-polar sites, which allows for multiple "Van der Waals forces" to occur, resulting in higher K/S values. The presence of alkyl chains in the structure of dyestuff made the materials more chemically alike and rely on the entropy of mixing, however, at 4%, the fabric was saturated and there is no longer increase in K/S.

Effect of system temperature on K/S of unmodified PP fabric dyed in scCO 2 medium.
The association between dye adsorption and system temperature (100,120, 130 °C) in scCO 2 was studied as shown in (Fig. 4). The experiment was carried out at a 3% concentration, a pressure of 25 MPa, a dyeing time of 60 min, and a dyeing medium flow rate of 7.500 ml/ min. It was inferred that increasing the system temperature from 100 to 130 °C increased the K/S of PP fabrics dyed in scCO 2 . Increasing temperature increased the vapor pressure of the solute which lead to an increase in solubility. Additionally, the structure of the PP underwent swelling during dyeing due to high temperature and pressure which helps penetration of dye into the fiber. Also, the melting temperature of PP for most cases is about 160 to 170 °C depending on the tacticity and crystallinity of the polymer. Therefore, at 130 °C chain mobility was increased, and consequently aided the dyeing process. The result obtained was in good agreement with our previous work 31 .
Effect of system pressure on K/S of unmodified PP fabrics dyed in scCO 2 medium. PP was dyed at a concentration of 3%, pressure varying from 15 to 25 MPa, a temperature of 130 °C, and a dyeing period of 60 min to investigate the effects of system pressure on the dye uptake of fibers. As shown in (Fig. 5), K/S values of dyed PP fabrics were improved by increasing system pressures to 15-25 MPa. Raising pressure increased the

Effect of dyeing time on K/S of unmodified PP fabric dyed in scCO 2 medium. At times ranging
from 60 to 180 min, the effect of dyeing time on the adsorption of the vinyl sulfone disperse reactive dye on PP fabrics was observed. PP fabrics were dyed at 130 °C, a pressure 25 MPa, a dyeing medium flow rate of 7.500 ml/ min. As presented in (Fig. 6), by increasing the time from 60 to 120 min, the K/S on PP fabric was increased, and it became constant at 180 min. This is due to low viscosity and high diffusivity of CO 2 in supercritical state. As a result, it is much easier to permeate fiber than water during the dyeing process in scCO 2 . PP swells because of the permeation of CO 2 , thus causing a decrease in percent crystallinity and order which in turn improved dyeing. Therefore, the K/S values of the dyed PP were enhanced by increasing time.   Table 1. which indicated that the dyes have good affinity to PP fabrics. Positive values of b* caused the color hues of dyes on PP fabrics to shift in a yellowish direction on the yellow-blue axis. The positive values of a* mean that the color hues of the dyes on PP fabrics changed in the redness direction on the red-green axis. Dyeing of unmodified PP fabric in scCO 2 , as a lower viscosity than water rendered of scCO 2 capable of penetrating unmodified PP fabric thoroughly, hence resulted in a higher dye uptake compared with that using aqueous method with 3% dye concentration. In addition, the synthesized dye molecule containing an aliphatic substituent group (N, N-diethyl), which increase the solubility of the synthesized dye in scCO 2 , linking the dye with the unmodified PP fabric via the "Van der Waals forces" 43,44 . A comparison of color assessment of scCO 2 and aqueous dyed fabrics showed that, without the addition of a carrier or dispersing agent, the appreciable color assessment of the dyed samples in scCO 2 was significantly higher than that of the dyed samples in water.
The levelling properties of dyed unmodified polypropylene fabrics in scCO 2 using the vinyl sulfone dye were illustrated in Table 1 from which it is clear that the average colour differences (ΔE, calculated from the CIE L*a*b* coordinates) of the dyed unmodified polypropylene fabrics indicated very good levelling properties in scCO 2 . This can be attributed to the fact that the structure of the unmodified polypropylene underwent swelling during scCO 2 dyeing due to high temperature and pressure which is helping in penetration of vinyl sulfone dye into the fiber.
Color fastness, along with color strength, plays an important role in dyeing performance. According to the geometric grey scale, all of the dyed PP fabrics in water and scCO 2 had excellent fastness values to washing, rubbing, and light, as shown in Table 2. The light fastness of samples dyed in scCO 2 medium showed a good value (rating was 4) on the blue scale, which was evident. The results of rubbing fastness showed good values (rating between 3-4 and 4). The results of washing fastness in both water and scCO 2 medium were outstanding. (rating between 4-5 and 5) as shown in Table 2.
Antibacterial activity. The antibacterial activity of the dyestuff under study and the dyed PP samples were investigated against Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and expressed as zone of growth inhibition ZI (mm). The antibacterial activity was determined by the agar well diffusion method given in Table 3 24 . All tested dyestuff and treated PP samples showed positive antibacterial activities against the examined bacteria. The antibacterial activity of the unmodified PP fabrics dyed in scCO 2 was significantly higher than that of the dyed fabrics in water. This can be attributed to the fact that the structure of the unmodified PP fabrics underwent swelling during scCO 2 dyeing due to high temperature and pressure which  www.nature.com/scientificreports/ helped penetration of more sulfonyl functional dye into the unmodified PP fabrics than that of the dyed fabrics in water. There was no discernible difference in the diameters of the inhibition zones for unmodified PP fabrics dyed in scCO 2 before and after five washing cycles, confirming the durability of the PP fabrics dyed in scCO 2 .
Mechanical testing of unmodified PP fabrics. The results of elongation at break and maximum force (N) of the dyed fibers under both water and scCO 2 medium were measured, and the results were reviewed in Table 4. After scCO 2 dyeing there was an increase in elongation by 1.5% compared to undyed PP, while the sample dyed under water showed a decrease in elongation with a value of 8.5%. This was attributed to the fact that increasing temperature in water in presence of dispersing agent caused degradation of the polypropylene fabrics 26 Alternatively, the sample dyed under scCO 2 revealed a little decrease in maximum force by 7.4% while the sample dyed under water suffered from a 12% decrease. Ultimately, dyeing PP under scCO 2 medium was better than dyeing in water medium because scCO 2 dyeing maintained the physiochemical properties of the fiber.
Raman spectra. Figures 7 and 8 showed the Raman spectrum and pictures of undyed and dyed PP. A Raman spectrum for undyed PP indicated several peaks. First set of peaks were observed between 900 and 1600 cm -1 . The rest of peaks were located between 2700 and 4000 cm -1 . The similar trend of PP spectrum was studied by Bhattacharyya et al. 42 .
The vinyl sulfone disperse reactive dye under study was characterized by Raman analysis and to make sure that the dyeing happened to have absorption inside or on surface of the PP fabrics. A comparison of Raman spectra for dyed and undyed PP fabrics was given in Fig. 7. Spectra were collected in the range of (100 to 4000 cm -1 ). The spectra were acquired using scan time settings of (3 s) and a resolution of 2.32 cm -1 / pixel for fiber analysis.  www.nature.com/scientificreports/ (SO 2 ). The recorded Raman spectra confirmed that all the designed and expected characteristic groups were implicated in the chemical structure of the vinyl sulfone disperse reactive dye.

Conclusion
A facile one step dyeing and finishing process was constructed to dye unmodified PP fabrics with antibacterial disperse reactive dye in scCO 2 .The results showed that, the synthesized antibacterial vinylsulfone disperse reactive dye have high affinity for dyeing PP fabrics in water and scCO 2 . The K/S was enhanced using scCO 2 . The ratings of fastness to washing, light and rubbing ranged from very good to excellent with the increase of the depth of shade. The optimum dyeing conditions for dyeing PP in scCO 2 were set to be 130 °C, pressure 25 MPa, dyeing concentration 3% at 120 min, based on the results of color strength, color fastness, and mechanical properties. Therefore, dyeing of PP fabric under scCO 2 medium was proved to be effective and potential for industrial applications.

Data availability
All data generated or analyzed during this study are included in this published article [and its supplementary information files].